Skip to main content
. 2013 Oct 20;19(12):1305–1320. doi: 10.1089/ars.2012.5021

FIG. 5.

FIG. 5.

MALDI-TOF mass analysis of C110-containing peptides from H3.2/H3.3 fraction using nonreducing and reducing conditions. Cysteine 110 from H3 isoforms H3.2 and H3.3 binds to GSH. H3.2/H3.3 fractions were obtained by RP-HPLC (A) Evaluation of 2582.4 m/z (BioGEE-H3.2/H3.3; peptide 106–122; DTNLCBioGEEAIHAKRVTIMPK) peak intensity before and after reduction of BioGEE-H3.2/H3.3 sample. Upper: nonreduced BioGEE sample; Lower: BioGEE reduced sample. (B) Evaluation of 1911 m/z (peptide 106–122; DTNLCAIHAKRVTIMPK) peak intensity before and after reduction of BioGEE-H3.2/H3.3 sample. Upper: nonreduced BioGEE sample; Lower: BioGEE reduced sample.