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. 2013 Oct 20;19(12):1305–1320. doi: 10.1089/ars.2012.5021

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 6. — Age-related changes of post-translational modifications in SAMP8 and C57BL/6J mice models. Decreased acetylation of H3K9 when glutathionylation of H3 decreases in livers from SAMP8 and old C57BL/6J mice. Age-related changes of glutathionylated histone H3 and acetyl and tri-methyl histone H3 in Lys9 (H3K9ac and H3K9me3) determined by immunoblot in liver isolated histones obtained from (A) SAMP8 strain (2 and 11 months) and (B) C57BL/6J strain (3 and 20 months). Histones were acid extracted from liver tissue following the procedure described in methods and separated in a 15% SDS-PAGE electrophoresis using non reducing conditions. Histone H3 was used as loading control.