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. Author manuscript; available in PMC: 2013 Oct 5.
Published in final edited form as: Circulation. 2009 Jan 12;119(3):408–416. doi: 10.1161/CIRCULATIONAHA.108.822072

Figure 4. Contractile function, calcium handling, and electrophysiological properties of isolated cardiomyocytes from WT and PDE5-TG mice.

Figure 4

Examples of cell shortening during 1-Hz field-stimulation confirm comparable fractional shortening normalized to resting cell length, L/L0, of paced cardiomyocytes (mean 7.6% in WT versus 7.3% in PDE5-TG, >60 cells per group, A). Contraction, indicated as percent cell shortening, significantly increased following isoproterenol stimulation in both genotypes (n=13 and 16 for WT and PDE5-TG) (B). Following PDE5 inhibition, contraction is modestly reduced compared to baseline and enhanced again after β-adrenergic stimulation; relative changes are similar in WT (n=5) and PDE5-TG cardiomyocytes (n=5) (C). Amplitude and voltage dependence of L-type calcium channel current ICaL were similar in both genotypes (n=11 WT, n=11 PDE5-TG) (D). *P<0.05 for the effect of the intervention compared to baseline.