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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: J Am Chem Soc. 2013 Sep 3;135(36):13512–13520. doi: 10.1021/ja4059487

Fig. 5.

Fig. 5

Metal selectivity of ZBR1 in aqueous solution at pH 7.0. (50 mM PIPES, 100 mM KCl). For each sample, 5 μM ZBR1 was mixed with 50 μM metal ion of interest (light gray) and then subsequently treated with 50 μM ZnCl2 (dark gray). The integrated fluorescence (550 to 800 nm) after each addition was normalized to the fluorescence of the metal-free sensor. λex = 525 nm.