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. Author manuscript; available in PMC: 2013 Oct 5.
Published in final edited form as: J Am Chem Soc. 2012 Feb 16;134(8):3667–3670. doi: 10.1021/ja2117816

Figure 2.

Figure 2

15N and 13C R1ρ relaxation dispersion sense the same DNA transient states. (a) DNA constructs. (b) Chemical shift comparison between imino 15N (red) or sugar/base 13C (blue) of transient states (TS) with a trapped A•T HG base pair (1mA, green) average values for N1/N3 or as reported before1 for C8/C1’ from DFT calculations (DFT), in A6-DNA (A6) and A2-DNA (A2). (c) On-resonance R1ρ relaxation dispersion profiles showing coupled chemical exchange for 15N and 13C sites (insets) in A•T and G•C base pairs at the CA step and neighboring base pairs and absence of exchange in other residues (G11 and T22), highlighted as in b).