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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Cell Neurosci. 2013 May 25;0:201–211. doi: 10.1016/j.mcn.2013.05.003

Figure 1.

Figure 1

Rapid appearance of γ2-GABARs on surface membrane of HEK293 cells. Surface α-BT fluorescence in the HEK293 cells expressing α1β3t-γ2 or α1β3t-δ subunit-containing receptors. Following blockade of existing surface receptors with unlabeled α-BT, the cells were incubated at 37 °C for 30 or 60 min, and newly inserted receptors were detected by incubation with labeled α-BT (red). GFP fluorescence was used to detect transfected cells. Insets in each panel show red fluorescence of surface α-BT in single cells from each panel to highlight the differences.