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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Cell Neurosci. 2013 May 25;0:201–211. doi: 10.1016/j.mcn.2013.05.003

Figure 6.

Figure 6

The time course of insertion of δ-GABARs in cultured hippocampal neurons. A: Representative images showing IR of newly inserted δ-GABARs (red) over the cell soma and in the dendrites of neurons incubated at 37°C for 0, 30, 60, 120 and 180 min. Total surface expression of δ-GABARs is shown in the panel total surface. Surface δ subunit IR in the neurons incubated with brefeldin-A (5 μg/ml) for 60 min is also shown (BFA). Panels on the right show δ subunit IR over the dendrites. The white bar corresponds with 10 μm. MAP2 IR (green) was used to identify dendrites. Panels on the right are magnified portions of dendrites showing δ subunit IR. B: Surface δ subunit IR was normalized to the cell area and plotted as a function of time (gray). A single-phase association equation best fit the data. n=25–30 neurons from 4–5 replicates per time point.