Table 1.
Receptor type | 5 min | 15 min | 30 min | 60 min | Total surface expressed |
---|---|---|---|---|---|
Native γ2- GABARs | 0.04 ± 0.02* | 0.085 ± 0.028* | 0.11 ± 0.03 | 0.09 ± 0.03* | 0.16 ± 0.05 |
α1β3t-γ2 | - | 0.035 ± 0.01* | 0.050 ± 0.007* | 0.051± 0.007* | 0.11 ± 0.005 |
α4β3t-γ2 | - | 0.027 ± 0.006* | 0.079 ± 0.011 | 0.09 ± 0.009 | 0.15 ± 0.02 |
30 min | 60 min | 120 min | 180 min | Total surface expressed | |
Native δ-GABARs | 0.04 ± 0.02* | 0.08 ± 0.03* | 0.11 ± 0.02* | 0.13 ± 0.06* | 0.20 ± 0.04 |
α1β3t-δ | 0.023 ± 0.004* | 0.024 ± 0.005* | 0.035 ± 0.005* | 0.041 ± 0.007* | 0.097 ± 0.01 |
α4β3t-δ | 0.018 ± 0.004* | 0.033 ± 0.005 | 0.043 ± 0.008 | 0.042 ± 0.004 | 0.077 ± 0.01 |
Insertion of native receptors expressed in cultured hippocampal neurons and recombinant receptors expressed in HEK293 cells. Values represent surface immunoreactive area normalized to the cell area, mean ± SE from 30–36 cells/ 4–7 replicates each.
p<0.05 vs total surface expressed receptors.