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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Cell Neurosci. 2013 Jul 3;0:272–282. doi: 10.1016/j.mcn.2013.06.005

Figure 8.

Figure 8

STAT3 interacts with the neuronal cytoskeleton. (A) Dissociated neurons were pretreated with DMSO vehicle (Veh) or 20μm Stattic and then stimulated with NGF (100ng/ml) and CNTF (150ng/ml). STAT3 was immunoprecipitated with STAT3 Ab sepharose bead conjugate or isotype control sepharose bead conjugate IgG control (IgG). 10% input of lysate is shown. βIII-Tubulin (Tub) and STAT3 were identified by western blot. Tubulin was precipitated together with STAT3. (left panel) Optical Density (O.D.) quantification of immunoprecipitated βIII-Tubulin averaged from 3 independent experiments. Data are mean ±SEM, *p<0.05.