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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Pain. 2013 May 31;14(10):1031–1044. doi: 10.1016/j.jpain.2013.03.012

Figure 3.

Figure 3

Paclitaxel induces expression of CCR2 in DRG but not SDH. A. Immunostaining shows marked upregulation of CCR2 in DRG neurons from paclitaxel- but not vehicle-treated or naïve animals. Pre-absorption of CCR2 blocked the immunohistochemical staining of DRG. B. The increase of CCR2 is observed as early as 4 hours and persists through 28 days after treatment. C. CCR2+ (red) neurons are co-labeled (yellow) with NF200 (green) but not with CGRP (green) or IB4 (green). D. No signal of CCR2 is detected by immunostaining in SDH after either paclitaxel or vehicle treatment. E. Paclitaxel induces an increase in mRNA of CCR2 in DRG (F[2,34] = 40.05, p < 0.0001, two-way ANOVA, n = 4, 8 and 8 for naïve, paclitaxel or vehicle at both time points) but not SDH at 4 hours and 7 days following treatment. * p < 0.0001 versus vehicle; # p < 0.001 versus naïve. Scale bar: 100 um (A and C) and 200 um (D).