Figure 5.

Diagnostic Markers of Normal Human Skin in Comparison to Regenerated Human TG1-Deficient Skin before and after Application of rhTG1 LUV Preparations

Normal human skin showed a pericellular distribution of TG1 activity in the granular layer of the epidermis (A). Cholesterol clefts in the stratum corneum were not present (F). Untreated human skin grafts and grafts treated with a retinoid cream showed cholesterol clefts within the stratum corneum (G and H), whereas TG1 activity was absent (B and C). After treatment of regenerated skin grafts with rhTG1 LUV formulations, the epidermis normalized: TG1 activity was detected with both dosages (D and E), and the formation of cholesterol clefts vanished (I) or was markedly reduced (J). “CC” stands for cholesterol cleft.