Table 1.
No. | Structure | HPLC (min) | MS calc./found (M + H) + | Ki (nM)b |
---|---|---|---|---|
1 | Phac-Arg-Val-Arg-4-Amba | 24.40 | 678.4/679.4 | 0.81 |
2 | Acetyl-Val-Arg-4-Amba | 18.58 | 446.3/447.3 | 2390 |
3 | Biotin-Adoa-Val-Arg-4-Amba | 24.62 | 775.4/776.5 | 4200 |
4 | Phac-Arg-Pro-Arg-4-Amba | 22.77 | 676.4/339.2a | 36.7 |
5 | Biotin-Adoa-Arg-Pro-Arg-4-Amba | 21.36 | 929.5/930.6 | 38.3 |
6 | Biotin-(Adoa)2-Arg-Pro-Arg-4-Amba | 22.82 | 1074.6/1075.5 | 57.4 |
7 | Biotin-(Adoa)3-Arg-Pro-Arg-4-Amba | 24.07 | 1219.7/611.1a | 15.1 |
8 | Phac-Arg-Thr-Arg-4-Amba | 21.40 | 680.4/341.3a | 6.0 |
9 | Biotin-(Adoa)2-Arg-Thr-Arg-4-Amba | 22.53 | 1078.6/540.5a | 9.0 |
(M+2H)2+/2.
Ki measurements were performed as described previously (Becker et al., 2010; Sielaff et al., 2011).