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. 2013 Aug 13;32(19):2561–2574. doi: 10.1038/emboj.2013.177

Figure 1.

Figure 1

Absence of Stat3 does not alter ES cell identity, pluripotency, or self-renewal in 2i. (A) GFP-labelled Stat3−/− ES cells were injected into blastocyst stage embryos; embryos were scored at E9.5 for the presence of GFP-positive cells and 15 embryos out of 19 showed widespread chimaerism. At mid-gestation (E12.5), 3 out of 3 embryos showed widespread chimaerism (not shown). A representative chimaeric embryo at E9.5 is shown. The embryo on the right showed no chimaerism and serves as a control for autofluorescence. See also Supplementary Figure S1A. (B) Absolute expression levels measured by RNA sequencing of genes associated with pluripotency or lineage priming, in Stat3+/+ and Stat3−/− cells. RPKM (Reads Per Kilobase per Million mapped reads) is a normalized unit of mRNA expression. (C) Morphology of Stat3+/+ and Stat3−/− cells in 2i culture. Both cell lines show homogeneous morphology with no sign of spontaneous differentiation. Scale bar, 100 μm. (D) Clonogenicity assay on Stat3+/+ and Stat3−/− cells. Three hundred cells per well were plated in 2i on laminin-coated plates and stained for alkaline phosphatase (AP) after 5 days. Bars show the number of AP-positive colonies obtained. Mean and s.d. of two independent experiments is shown.