Figure 5.

SIRT2 deacetylates NF-κB p65 subunit at Lys310 in microglia. (A) Western blot analysis of endogeneous NF-κB p65 acetylation at Lys310 in CTR and SIRT2 KD N9 cells. * Indicates a non-specific band previously described for this antibody (Rothgiesser et al, 2010a). (B) Quantification of mpa2l mRNA levels showed increased transcription in activated microglia lacking SIRT2. Data shown are presented as mean±s.d. and representative of three independent experiments done in triplicate, two-way ANOVA, **P<0.01. (C) Levels of NF-κB p65 acetylation at Lys310 in SIRT2 KD cells viral-transduced cells with CTR, SIRT2, and SIRT2 S331 phosphorylation mutants (S331A and S331D) analysed by western blotting. The blot is representative of three independent experiments. (D) Acetylation of NF-κB p65 at Lys310 in microglia and mixed primary cultures analysed by western blotting.

Source data for this figure is available on the online supplementary information page.

Source Data for Figure 5C ^{(542.5KB, pdf)}

Source Data for Figure 5D ^{(211.6KB, pdf)}