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. 2013 Oct 7;6:31. doi: 10.3389/fnmol.2013.00031

Figure 5.

Figure 5

Characterization of EP1 receptor mediated changes in [Ca2+]i in neurons. In a separate set of neuronal cultures, neurons were pre-treated with SC-51089 (10 μM), NS-398 (10 μM), MK-801 (10 μM), nifedepine (10 μM), ryanodine (50 μM) or vehicle control before 17-pt-PGE2 (100 nM) application and fluorescence intensity was recorded. Bars above graphs indicate application time of 17-pt-PGE2 and antagonists/vehicle control. (A–E) Δ F/F0 and changes in AUC induced by 17-pt-PGE2 alone and following pre-treatment with (A) SC-51089, (B) NS-398, (C) MK-801, (D) nifedepine, and (E) ryanodine. Data represent mean ± SEM. Statistical analysis was carried out using One-Way ANOVA with Bonferroni's multiple comparison tests. ***p < 0.001 for17-pt-PGE2 alone or 17-pt-PGE2 plus antagonist. **p < 0.01 and *p < 0.05 for 17-pt-PGE2 plus antagonist vs. antagonist alone; ns, not significant.