Figure 5.

Effects of Treatment of G361 Cells with AMPK Activators on Phosphorylation of ACC, Phosphorylation and Activity of AMPK, Cellular ADP:ATP Ratios, and Calculated Cellular AMP

(A–C) Effects of treatment of G361 cells with AMPK activators on phosphorylation of ACC, phosphorylation and activity of AMPK (A), cellular ADP:ATP ratios (B), and calculated cellular [AMP] (C). Replicate dishes of cells (n = 3) were incubated for 1 hr with A23187 (10 μM), berberine (100 μM), A769662 (100 μM), or an equivalent concentration of vehicle (DMSO) and then lysed in parallel in neutral detergent buffer for measurement of AMPK activity in immunoprecipitates (top) or western blotting (bottom), or in perchloric acid for analysis of ADP:ATP ratios (center). AMP concentrations (see Table S1) were calculated as described in Experimental Procedures. Data are mean ± SD. Significantly different from DMSO control: ^∗∗∗p < 0.001.