Table 1.
EC50 values for the rate and extent of aggregation induced by collagen-related peptide (CRP), collagen and U46619 in wild-type (WT), adhesion and degranulation-promoting adapter protein-deficient (ADAP−/−) and Fc receptor γ-chain-deficient (FcRγ−/−) platelets
| CRP (μg mL−1) |
Collagen (μg mL−1) |
U46619 (μM) |
|
|---|---|---|---|
| Rate of aggregation: mean EC50 value (95% CI) | |||
| WT (n = 9) | 1.1 (0.78–1.6) | 1.0 (0.83–1.2) | 0.43 (0.28–0.66) |
| ADAP−/− (n = 9) | 1.4 (1.1–1.7) | 2.6 (1.7–3.9) | 0.68 (0.42–1.1) |
| FcRγ−/− (n = 5) | NR | NR | 0.51 (0.27–0.94) |
| P-value | 0.21* | < 0.0001* | 0.06† |
| Extent of aggregation: mean EC50 value (95% CI) | |||
| WT (n = 9) | 0.63 (0.39–1.0) | 0.89 (0.78–1.0) | 0.38 (0.25–0.57) |
| ADAP−/− (n = 9) | 0.69 (0.44–1.1) | 2.1 (1.8–2.5) | 0.58 (0.37–0.93) |
| FcRc−/− (n = 5) | NR | NR | 0.45 (0.29–0.68) |
| P-value | 0.71* | < 0.00001* | 0.01† |
CI, confidence interval; NR, not recorded. Statistical comparison of pA50 values (− log EC50) was carried out with paired
*
t-tests
†
or anova.