Table 1.

Primer Pairs and Annealing Temperatures Used to Amplify the PTPN11 Coding Sequence and Sizes of PCR Products

	Primer Sequence(5′→3′)
Exon	Forward	Reverse	Annealing Temperature(°C)	Product Length(bp)
1	GCTGACGGGAAGCAGGAAGTGG	CTGGCACCCGTGGTTCCCTC	60	589
2	ACTGAATCCCAGGTCTCTACCAAG	CAGCAAGCTATCCAAGCATGGT	60	405
3	CGACGTGGAAGATGAGATCTGA	CAGTCACAAGCCTTTGGAGTCAG	60	384
4	AGGAGAGCTGACTGTATACAGTAG	CATCTGTAGGTGATAGAGCAAGA	58	447
5	CTGCAGTGAACATGAGAGTGCTTG	GTTGAAGCTGCAATGGGTACATG	60	329
6	TGCATTAACACCGTTTTCTGT	GTCAGTTTCAAGTCTCTCAGGTC	54	282
7	GAACATTTCCTAGGATGAATTCC	GGTACAGAGGTGCTAGGAATCA	56	271
8	GACATCAGGCAGTGTTCACGTTAC	CCTTAAAGTTACTTTCAGGACATG	57	350
9	GTAAGCTTTGCTTTTCACAGTG	CTAAACATGGCCAATCTGACATGTC	56	357
10	GCAAGACTTGAACATTTGTTTGTTGC	GACCCTGAATTCCTACACACCATC	60	284
11	CAAAAGGAGACGAGTTCTGGGAAC	GCAGTTGCTCTATGCCTCAAACAG	60	453
12	GCTCCAAAGAGTAGACATTGTTTC	GACTGTTTTCGTGAGCACTTTC	56	250
13	CAACACTGTAGCCATTGCAACA	CGTATCCAAGAGGCCTAGCAAG	60	356
14a	ACCATTGTCCCTCACATGTGC	CAGTGAAAGGCATGTGCTACAAAC	60	259
15	CAGGTCCTAGGCACAGGAACTG	ACATTCCCAAATTGCTTGCCT	60	321

^aGC clamps were added at the 5′ end for DHPLC analysis: forward primer, 5′-CCCGCCGCCCCCGCCG-3′; reverse primer, 5′-CCGCGCCCCCGCCCG-3′ (product length = 290 bp).