Table 2.

Percent Buffer B and Temperatures Used in DHPLC Analysis for PTPN11 Mutation Detection

	% Buffer Ba
Exon	Loading	Initial	Final	Temperature(s)(°C)
1	56	61	67	67
2	55	60	66	56, 57
3	54	59	65	57, 58
4	53	58	64	56, 57
5	51	56	62	56, 58
6	50	55	61	56, 57
7	50	55	61	56, 57
8	51	56	62	57, 58
9	52	57	63	56, 57
10	50	55	61	57, 58
11	54	59	65	59
	49	54	60	64
12	48	53	59	58, 59
13	51	56	62	59
	50	55	61	60
14	52	57	63	57
	49	54	60	60
15	51	56	62	56, 57

^a% bufferA=100-% buffer B.