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. 2013 Oct 7;3:2873. doi: 10.1038/srep02873

Figure 7. Competition ELISA.

Figure 7

Spectrin and F-actin were pre-incubated with increasing doses of rBjP4.1 (a), U2/3 (b) or FA (c), and allowed to react with recombinant human 4.1R immobilized on a 96-well ELISA plate. In addition, spectrin and F-actin were also pre-incubated with increasing doses of human 4.1R (d), and allowed to react with U2/3 immobilized on a 96-well ELISA plate. Bound spectrin or actin was detected as described under “Methods”, and the levels were expressed as a percentage of binding in the absence of any compound.