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. 2013 Sep 20;4:2443. doi: 10.1038/ncomms3443

Figure 7. ERAP suppresses growth of TAM-R tumours.

Figure 7

(a,b) Immunoblot analyses were performed to evaluate the effects of ERAP on the phosphorylation levels of Akt, p42/44 MAPK and ERα proteins in TAM-R MCF-7 (a), and a parent T47D (T47D-WT) and TAM-R T47D (b) cells. (c) MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were performed to evaluate the inhibitory effect of ERAP on the growth of TAM-R MCF-7 cells. TAM-R MCF-7 cells were treated for 24 h with E2±ERAP in the presence or absence of 1 μM tamoxifen. The data represent the mean±s.e.m. of three independent experiments (*P<0.05, ***P<0.001 in two-sided Student’s t-test). (d) Inhibitory effect of ERAP on the growth of T47D-WT (upper) and TAM-R T47D (lower) cells after 24 h of treatment with E2 alone or E2 and IGF-2 in the presence and the absence of 10 nM tamoxifen, respectively. The data of all panels represent the mean±s.e.m. of three independent experiments (*P<0.05, **P<0.01, ***P<0.001 in two-sided Student’s t-test). (e) ERAP inhibits the tumour growth of TAM-R MCF-7 orthotropic breast cancer xenografts in nude mice. The tumour volume represents the mean±s.e.m. of each group (n=5) (*P<0.05, ***P<0.001 in two-sided Student’s t-test). (f) ERAP inhibits the tumour growth of both T47D-WT (left) and TAM-R T47D (right) orthotropic breast cancer xenografts in nude mice. The tumour volume represents the mean±s.e.m. of each group (n=5; *P<0.05, **P<0.01, ***P<0.001 in two-sided Student’s t-test).