Figure 2.

Pattern of cross-species residue conservation in NOD1. Residues conserved across all NOD1 species checked, or just across mammals, are highlighted green and purple respectively. Residues are mapped onto the amino acid sequence for human NOD1. The domain architecture is highlighted underneath the relevant stretch of sequence as follows: CARDs – gold; NACHT – dark blue; C-rich region – blue; Winged-helix – pale blue; LRRs – red. The motifs responsible for RIP2 binding, the Walker A and B motifs and the Sensor 1 region are all labeled in black above the relevant sequence. Also labeled above the sequence are LxxLL motifs [black bars (L1, L2)], residues predicted to be important for ubiquitin binding (purple asterisks), and residues predicted to be involved in ligand recognition (blue asterisks).