Figure 3.

Pattern of cross-species residue conservation in NOD2. Residues conserved across all NOD2 species checked, or just across mammals, are highlighted green and purple respectively. Residues are mapped onto the amino acid sequence for human NOD2. The domain architecture is highlighted underneath the relevant stretch of sequence as follows: CARDs – gold; NACHT – dark blue; C-rich region – blue; Winged-helix – pale blue; LRRs – red. The motifs responsible for RIP2 binding, the Walker A and B motifs, the Sensor 1 region and the regulatory region from residues 664 to 854 are all labeled in black above the relevant sequence. Also labeled above the sequence are the LxxLL motifs [black bars (L1, L2, L3, L4)], residues predicted to be important for interaction with RIP2 (yellow asterisks), ubiquitin binding (purple asterisks), and residues predicted to be involved in ligand recognition (blue asterisks).