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. 2013 Sep 27;4:2501. doi: 10.1038/ncomms3501

Figure 5. A single residue determines cold sensitivity of r- and mTRPA1.

Figure 5

(a) Sequence alignment of S5 and S5-S6 linker domains of r-and hTRPA1. The critical residues G878 (rTRPA1) is marked by *. (b,c) rTRPA1-V893I/F897L/A900P/T903S/L908I but not rTRPA1-G878V retained cold sensitivity in Ca2+ assay (n=12) and single-channel recordings (n=4). Scale bars are 10 pA and 25 s (left) and 10 pA and 50 s (right). (d) Representative single-channel recordings of mTRPA1/G878V in responses to cold and AITC (100 μM). Scale bars are 4 pA and 50 s. (e) Relative activity of wild type and mTRPA1/G878V, as determined by normalizing NPo against AITC response. n=5. *P<0.05 from Student’s t-test. Error bars are s.d.