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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Anal Chem. 2013 Aug 20;85(17):8046–8054. doi: 10.1021/ac401795y

Table 1.

Primer Sequences

Primer Sequence (5’-3’)
UL28-Xho1-F CTTTGCCTCGGTCTACCGGTGCGGGG
gBΔSS-BglII-R GGGAGATCTGAGGCGGGACTACGGGGGCCCGTCG
gB-797-BglII-F GGGAGATCTGGGTGGAGGTGGAGGTTACGTCATGCG GCTGCAGAGCAAC
gB-nc-BamH1-R GGGATCCCAACCGGAGGCATCCAAC
gB-CD4-BglII-F GGCAGATCTACCATGAACCGGGGAGTCCCTTTTAGG
gB-CD4V5-BglII-R CCCAGATCTCTACGTAGAATCTAGACCGAGGAGAGGGTTAGGGATAGGCT-
TACCAATGGGGCTACATGTCTTCTGAAA
gB-GPR77-BglII-F GGCAGATCTACCATGGGGAACGATTCTGTCAGCTAC
gB-GPR77V5-BglII-R CCCAGATCTCTACGTAGAATCTAGACCGAGGAGAGGGTTAGGGATAGGCT-
TACCAATGGGGCTACATGTCTTCTGAAA
gC-KAN-F CGCTTTGCCGGGAACGCTAGCCGATCCCTCGCGAGGGGGAGGCGTCGGGC-
GGCCTGGTGATGATGGCGGGATCG
gC-KAN-R GGGGGGACCAAACTATATAGATATTAAAAAGGTAACGGGGGGGTCTTGCG-
TCAGAAGAACTCGTCAAGAAGGCG
CD4-F GGAATTCAACATGAACCGGGGAGTCCCTTTTAGG
CD4-R-Overlap CCCGATTCCAATTGGCTGCACCGGGGTGGACCATGT
GPR77-F GGAATTCACCATGGGGAACGATTCTGTCAGCTAC
GPR77-R-Overlap CCCGATTCCAATGGGTTCAGCCCGCAGGGCCCTGGC
gC-F-Overlap-CD4 CCGGTGCAGCCAATTGGAATCGGGGTTCTCGCGGCG
gC-F-Overlap-GPR77 CGGGCTGAACCCATTGGAATCGGGGTTCTCGCGGCG
gC-R GGGGATCCTTACCGCCGATGACGCTGC
gC-RedET(CD4)-F CGCTTTGCCGGGAACGCTAGCCGATCCCTCGCGAGGGGGAGGCGTCGGGC-
ACCATGAACCGGGGAGTCCCTTTTAGG
gC-RedET(GPR77)-F CGCTTTGCCGGGAACGCTAGCCGATCCCTCGCGAGGGGGAGGCGTCGGGC-
ACCATGGGGAACGATTCTGTCAGCTAC
gC-RedET-R GGGGGGACCAAACTATATAGATATTAAAAAGGTAACGGGGGGGTCTTGCG-
TTACCGCCGATGACGCTGCCGCGA