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. 2013 Oct;183(4):1339–1350. doi: 10.1016/j.ajpath.2013.06.027

Figure 4.

Figure 4

Mammary glands from Dmp1+/+;MMTV-cyclin D1 and Dmp1+/+;MMTV-D1T286A mice show lower proliferation and increased Arf/Ink4a expression. A: Mammary tissues derived from MMTV-cyclin D1 and Dmp1+/+;MMTV-D1T286A mice showed increased proliferation at Dmp1 heterozygous background. Representative images of Ki-67 immunostaining (red) in mammary glands from Dmp1+/+; and Dmp1+/−; MMTV-cyclin D1, and MMTV-D1T286A mice. Nuclei were counterstained with hematoxylin (blue). Scale bars: 100 μm. Quantification of Ki-67–positive cells per mammary gland section is shown. Error bars indicate means ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01. B–D: Real-time PCR analysis of Dmp1, p19Arf, and p16Ink4a mRNA levels in mammary glands from MMTV-cyclin D1 and MMTV-D1T286A mice in Dmp1+/+, Dmp1+/−, and Dmp1−/− backgrounds. Data were normalized against β-actin mRNA levels. Gray columns represent the samples from MMTV-cyclin D1 mice and black columns represent the samples from MMTV-cyclin D1T286A mice. N, normal mammary gland from nontransgenic mice.