Table 1.
PCR Products Used to Identify the Inversion Breakpoint on 17q21.3
| Primer | Sequence 5′→3′ | Position on Human PAC RP5-1029K10a | Productb (size in bp) | Present on Chimpanzee BAC RP43-134L13c | FISH Signal onChimpanzeeChromosome |
| p61 | TCAAGGCGGTGGGGAAGAGA | 35594 | p61/19 | − | |
| p19 | AATCTAGACTGGAGGCCACATT | 35849 | (255) | ||
| p18 | GGGCCCTCCTGAAACTTACA | 29299 | p18/19 | − | 19p |
| p19 | AATCTAGACTGGAGGCCACATT | 35849 | (6550) | ||
| p59 | GGAGAAGAGGCCCTGCACACC | 38650 | p59/60 | − | |
| p60 | ATCCCCTTTCTTCTCTCCCTT | 39259 | (609) | ||
| p58 | GATTCATCTCTGCAGCCTCC | 40603 | p58/p38 | − | |
| p38 | CCGTGTGGAAGTGGATCTGAGT | 40738 | (135) | ||
| p37 | CACTCAGTCCTGCCCTCTTC | 34866 | p37/38 | − | 19p |
| p38 | CCGTGTGGAAGTGGATCTGAGT | 40738 | (5872) | ||
| p51 | CCTGCCTTAGGCTTAACC | 42123 | p51/52 | − | |
| p52 | AACTCTTCCCCCATCCAC | 42377 | (254) | ||
| p42 | TGCCTTCCCTGCAGAACTCCA | 43328 | p42/43 | − | |
| p43 | GAGAGGTAATTCTGTGTCC | 43550 | (222) | ||
| p45 | CGCCTTCCTGCGTAAGATAG | 43962 | p45/48 | − | |
| p48 | ACAGCAGAGCACGGAGGAA | 44214 | (252) | ||
| p62 | TCTTAATTGCTGACACTTGT | 48116 | p62/63 | − | |
| p63 | AAGGCAGTTGCCATTTGGTT | 48217 | (101) | ||
| p40 | ACCAGTAGTCGTGTCAAATA | 51178 | p40/41 | − | |
| p41 | CTGACGCAAAGGACAAAATA | 51809 | (631) | ||
| p12 | AAGTTTAGACTGCCAGGA | 58652 | p12/65 | + | |
| p65 | GTGACACTCAGACCAAGCCC | 59187 | (535) | ||
| p12 | AAGTTTAGACTGCCAGGA | 58652 | p12/13 | + | 19q |
| p13 | GCCTGTGGGGCAGAAGTGGA | 63717 | (5065) | ||
| p14 | CTCTGAGAGGAGCTGGCAGT | 66018 | p14/p15 | + | 19q |
| p15 | AGCCCCCTTTGCCCCACTCCA | 71331 | (5313) | ||
| p1 | CAAAAGGGGTGGAGGAAGGAA | 82020 | p1/17 | + | |
| p17 | GATCGCATCTCTGGAAATAGTC | 82790 | (770) | ||
| p1 | CAAAAGGGGTGGAGGAAGGAA | 82020 | p1/7 | + | 19q |
| p7 | ACAGCAGGGTGCACAATAGA | 85362 | (3342) | ||
| p3 | CCACAGGACTTGGGCAGGC | 92498 | p3/4 | nd | 19q |
| p4 | CCTGTCCACACCAGGAGCAAG | 99569 | (7071) | ||
| p5 | TTACGTAGCTATCTTCCCAA | 111880 | p5/10 | nd | 19q |
| p10 | GCTATTTGGCCAGCCTGTGTA | 114438 | (2558) | ||
| p5 | TTACGTAGCTATCTTCCCAA | 111880 | p5/6 | nd | 19q |
| p6 | GATGATTTCCCACTTTAGC | 123509 | (11629) |
a
Numbering according to the sequence of PAC RP5-1029K10 available for GenBank accession number AC006487.
b
All PCR products were amplified from DNA of PAC RP5-1029K10.
c
− = Absent; + = present; nd = not done.