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. 2013 Sep 17;110(40):16175–16180. doi: 10.1073/pnas.1310432110

Fig. 5.

Fig. 5.

HPV16 E7 induces p16INK4A addiction. p16INK4A was depleted in (A) HFKs expressing control vector, HPV16 E7, E6, or E6 and E7; and (B) U2OS-tet on cells with doxycycline-inducible expression of HPV16 E7. Three independent p16 shRNA constructs (shp16AB, shp16CD, shp16EF) were used in A and two in B. (C) p16INK4A was depleted in U2OS-tet on cells with doxycycline-inducible expression of HPV16 E7. Two independent p16 shRNA constructs (shp16AB, shp16CD) and their respective rescue constructs (p16, 16CD) were used. (D) KDM6B was depleted in U2OS-tet on cells with doxycycline-inducible expression of HPV16 E7 and rescued with p16INK4A. Cell viability was measured by AlamarBlue assay. Averages and SDs for three independent experiments are shown. Statistically significant changes are indicated: *P < 0.05, **P < 0.01.