Table 1.
Degradation of peptide substrates by OOP and PreP1 analyzed by MS
| Peptide | Description/source | Length, aa | Isoelectric point* | Cleavage by OOP | Cleavage by PreP1 |
| pF1β 4–15 | Presequence fragment | 12 | 12 | RL↓L↓A↓SL↓L↓RQSAQ | R↓L↓LASL↓L↓R↓QSAQ |
| N. plumbaginifolia | |||||
| pF1β 43–53 | Presequence fragment | 11 | 9.99 | KGFL↓L↓N↓RAVQY | KG↓FLL↓NR↓AVQY |
| N. plumbaginifolia | |||||
| pL29 | Presequence | 19 | 12.48 | MFL↓TRFVG↓R↓RF↓L↓AAASARS | MFLT↓R↓FVGRR↓FLAAAS↓ARS |
| A. thaliana | |||||
| pACD1 | Presequence | 20 | 12.18 | MRGR↓S↓LTL↓S↓RVKLELARRSM | MRGR↓SLTLS↓R↓VKLEL↓A↓R↓R↓SM |
| A. thaliana | |||||
| tpACS 1–11 | Transit peptide | 11 | 6.50 | MSS↓N↓SL↓R↓HVE↓S | MSSNS↓LRHVES |
| A. thaliana | |||||
| tpACS 1–19 | Transit peptide | 19 | 6.50 | MSSNSLRHVESM↓S↓Q↓LP↓S↓GA | MSSNS↓LR↓HV↓E↓S↓MSQLP↓SGA |
| A. thaliana | |||||
| tpACS 1–26 | Transit peptide | 26 | 8.52 | MSSNSLRHVESMSQLPSGAGKISQLN | MSSNS↓LRHVES↓MSQLPSGAG↓KISQLN |
| A. thaliana | |||||
| pThrRS 1–19 | Presequence | 19 | 8.52 | MASSH↓S↓LLF↓S↓SSFL↓SKPSS | MASSHS↓L↓L↓F↓S↓S↓S↓F↓L↓SKPSS |
| A. thaliana | |||||
| SytII 40–60 | Non-presequence – synaptotagmin | 21 | 4.94 | GESQE↓DMF↓AKLK↓DKFF↓N↓EINK | GESQEDMFAKLKD↓K↓FFNE↓IN↓K |
| Rattus norvegicus | |||||
| octMdh1 | Oct1 generated octapeptide | 8 | 5.52 | FS↓S↓TVA↓NP | FSSTVANP |
| S. cerevisiae | |||||
| octSdh1 | Oct1 generated octapeptide | 8 | 9.75 | FTSSAL↓VR | FTSSALVR |
| S. cerevisiae | |||||
| Cyt1p 294–309 | Non-presequence | 16 | 12.03 | GIKTRK↓FVF↓N↓PPKPRK | GIKTR↓K↓FV↓FNPPKP↓R↓K |
| S. cerevisiae | |||||
| pCox4 | Presequence | 23 | 12.01 | MLSLRQ↓S↓I↓RF↓F↓KPATRTLSSSRY | MLSLRQS↓I↓R↓F↓FKPAT↓RTLSSSRY |
| S. cerevisiae |
The cleavage sites were inferred from the data in Dataset S1.
*
Calculated using the ExPASy compute Mw/pI tool (http://web.expasy.org/compute_pi/)