Table 1.
Rad51 inactivation-induced NHEJ products
| Relevant genotype | WT* | rad52ΔKO | rad51ΔKO | rad51Δw |
| 5FOAR Leu+ transformants analyzed | 144 | 120 | 132 | 120 |
| I-SceI–resistant products (%) | 0 (0) | 1 (0.83) | 14 (11) | 12 (10) |
| TCGTAGGG ATAa|CAGGGTAATCGAG | 0 | 1 | 3 | 4 |
| AGCATCCC|TATt GTCCCATTAGCTC | ||||
| TCGTAGGG AtAA|CAGGGTAATCGAG | 0 | 0 | 1 | 2 |
| AGCATCCC|TaTT GTCCCATTAGCTC | ||||
| TCGTAGGG AtaA|CAGGGTAATCGAG | 0 | 0 | 4 | 2 |
| AGCATCCC|TatT GTCCCATTAGCTC | ||||
| TCGTAGGG Ataa|CAGGGTAATCGAG | 0 | 0 | 2 | 1 |
| AGCATCCC|Tatt GTCCCATTAGCTC | ||||
| TCGTAGGG ataa|CAGGGTAATCGAG | 0 | 0 | 3 | 1 |
| AGCATCCC|tatt GTCCCATTAGCTC | ||||
| TCGTAGggataa|cagggTAATCGAG | 0 | 0 | 0 | 1 |
| AGCATCcc|tattgtcccATTAGCTC | ||||
| AGAACAAaaacctcgtagggataa|c | 0 | 0 | 1 | 1 |
| TCTTGTTtttggagcatccc|tattg | ||||
| agggtaat(625)actccaaagaacaa | ||||
| tcccatta(625)tgaggtttcttgtt |
Seven types of I-SceI–resistant sequences are shown, as the inserted 25-bp sequence (except for the last sequence) including the I-SceI sequence (italicized letters) and the portion lost (lowercase letters with strikethrough) due to imprecise NHEJ. The vertical lines indicate the I-SceI cleavage. Underlined sequences are putatively base pair annealed during imprecise NHEJ (Fig. S1C). The numbers of each type of imprecise NHEJ are for each strain. ΔKO shows alleles lacking most of the coding region, and Δw shows those lacking the entire coding region, from the initiation codon to the last sense codon.
*
The data for the wild type are obtained from a previous report (13).