Table 2.
Oligonucleotides Used for Sequencing the Entire Human mtDNA[Note]
|
Primer Coordinates |
|||
| Template PCRID Number | Primer Direction | 5′ np | 3′ np |
| 1 | Forward | 371 | 390 |
| 1 | Forward | 811 | 829 |
| 1 | Forward | 1169 | 1188 |
| 1 | Forward | 1832 | 1851 |
| 2 | Forward | 2206 | 2225 |
| 2 | Forward | 2772 | 2789 |
| 2 | Forward | 3108 | 3127 |
| 2 | Forward | 3388 | 3408 |
| 2 | Forward | 3816 | 3835 |
| 3 | Forward | 4308 | 4325 |
| 3 | Forward | 4831 | 4847 |
| 4 | Forward | 5591 | 5610 |
| 4 | Forward | 6149 | 6168 |
| 4 | Forward | 6449 | 6465 |
| 4 | Forward | 6795 | 6811 |
| 5 | Forward | 7366 | 7384 |
| 5 | Forward | 7871 | 7890 |
| 5 | Forward | 8829 | 8845 |
| 5 | Forward | 9026 | 9042 |
| 6 | Forward | 9802 | 9821 |
| 6 | Reverse | 10107 | 10088 |
| 6 | Forward | 10356 | 10375 |
| 6 | Forward | 11141 | 11158 |
| 6 | Forward | 11464 | 11484 |
| 7 | Forward | 12190 | 12209 |
| 7 | Forward | 12530 | 12547 |
| 7 | Forward | 13001 | 13020 |
| 8 | Forward | 13595 | 13611 |
| 8 | Reverse | 13950 | 13932 |
| 8 | Reverse | 14810 | 14791 |
| 8 | Reverse | 15461 | 15442 |
| 9 | Forward | 15409 | 15428 |
| 9 | Forward | 15978 | 15997 |
| 10 | Forward | 16225 | 16244 |
| 10 | Reverse | 429 | 408 |
Note.— The annealing temperature for all reactions was 50°C.