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Phototaxis apparatus and automated machine vision larval postural analysis. (A) Schematic of phototaxis assay generating a checkerboard lightscape. A digital projector generates arbitrary spatiotemporal light stimuli. Larvae are placed on a 25-cm dish coated in agar, and their resulting behavior is visualized by infrared LEDs and recorded with a camera. (B) Machine vision software segments the larval tracks into runs and turns. Turns are composed of accepted and/or rejected head-sweeps.
