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. 2013 Sep 16;110(40):16091–16096. doi: 10.1073/pnas.1215934110

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Freely available online through the PNAS open access option.

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Fig. 1. — Reduced number of thymocytes and peripheral T cells in Nfat5^fl/fl, ^Lck-Cre mice. (A) Thymus size in control (WT), Nfat5^fl/fl, ^Lck-Cre (Upper), or Nfat5^fl/fl, ^CD4-Cre (Lower) mice. (Scale bar, 1 cm.) (B) Body, thymus, and spleen weight in WT and Nfat5^fl/fl, ^Lck-Cre mice. (C) Cell numbers in inguinal lymph nodes, thymus, and spleen in WT and Nfat5^fl/fl, ^Lck-Cre mice. Each circle in B and C represents a single mouse, and the horizontal lines show the mean. (D) Numbers of CD3, CD4, and CD8 cells in lymph nodes of WT and Nfat5^fl/fl, ^Lck-Cre mice (Left) and WT and Nfat5^fl/fl, ^CD4-Cre mice (Right) (mean ± SEM); n ≥ 6. Numbers of B220-positive, CD3-positive, CD4-positive, and CD8-positive (E) and IgM-positive, CD11b-positive, and CD11c-positive (F) splenocytes in WT and Nfat5^fl/fl, ^Lck-Cre mice (mean ± SEM); n = 8. *P < 0.05; **P < 0.01; ***P < 0.001.