Fig. 5.

Overlay of ssNMR spectra of triply labeled DADMe-ImmH and hPNP–[1′-¹⁵N, ¹³CH₂, 9-¹³C]DADMe-ImmH–PO₄ complex. Chemical shifts are referenced to the chemical shift of adamantine set to 40.5 ppm. The peaks at 103.0 ppm and 102.3 ppm correspond to the 9-¹³C label in bound and free DADMe-ImmH, respectively. The peaks at 47.4 and 45.8 ppm correspond to the ¹³CH₂ label in the two diastereomers of free protonated DADMe-ImmH, and the peak at 45.0 ppm corresponds to the ¹³CH₂ label in the sole diastereomer present in the hPNP—bound protonated DADMe-ImmH.