Table 1.
Kd (nM) | ||||
---|---|---|---|---|
[NaCl] | ka (×106 M−1s−1) | kd (×10−3 s−1) | Kinetic fit | Steady state |
50 mM | 7.1 ± 0.8 | 26 ± 2 | 3.7 ± 0.4 | 4.0 ± 0.2 |
100 mM | 4.5 ± 0.9 | 29 ± 3 | 6.5 ± 0.6 | 6.3 ± 0.6 |
200 mM | 2.4 ± 0.2 | 32 ± 6 | 13 ± 1.1 | 13 ± 0.5 |
300 mM | 1.5 ± 0.2 | 33 ± 4 | 22 ± 0.3 | 21 ± 1.0 |
Kinetic analysis was performed by global fitting of 1:1 binding model: ka=(d[AB]/dt)/([A]*[B]), kd=(−d[AB]/dt)/[AB] and Kd=kd/ka, where [A], [B] and [AB] are the concentrations of the immobilized DNA, PA, and complex, respectively. Errors listed are the standard errors for the global fit.