Table 2.
Single-channel properties of MscS family members
| Species | Name | Unitary conductance | Ion selectivity (PCl : PK) | Number of TMHsc | Identity in the pore-lining domain + upper vestibule to EcMscS, %d | References | |
|---|---|---|---|---|---|---|---|
| Prokaryotes | E. coli | EcMscL a | 3 nS 1 | Non-selective | 2 | - | Sukharev, 1994; Häse, 1995 |
| EcMscS | 1.2 nS 1/350 pS 5 | 1.2–3 | 3 | 100 | Levina, 1999; Sukharev, 2002 | ||
| EcMscK | 1 nS 1 | < EcMscS | 11* | 32 | Martinac, 1987; Li, 2002 | ||
| YjeP | 250–400 pS 1 | NR | 11* | 27 | Edwards, 2012 | ||
| YbdG b | 350–400 pS 1 | NR | 5* | 21 | Schumann, 2010 | ||
| YnaI | ~ 100 pS 1 | NR | 5* | 30 | Edwards, 2012 | ||
| YbiO | ~ 850 pS 1 | NR | 12* | 24 | Edwards, 2012 | ||
| S. pomeroyi | MscSP | 1.04 nS 2 | 1.4 | 3* | 49 | Petrov, 2013 | |
| T. tengcongensis | TtMscS | 134 pS 1 | 8.7 | 3 | 29 | Zhang, 2013 | |
| C. glutamicum | MscCG | 328 pS 2 | 0.3 | 4 | 29 | Börngen, 2010; Becker, 2012 | |
| M. jannashii | MscMJLR | 2 nS 3 | 0.2 | 5* | 41 | Kloda, 2001a,b | |
| MscMJ | 270 pS 3 | 0.16 | 5* | 36 | Kloda, 2001a | ||
| Eukaryotes | C. reinhardtii | MSC1 | 390 pS 4 | 7 | 5* | 32 | Nakayama, 2007 |
| A. thaliana | MSL10 | 103 pS 5 | 5.9 (PCl : PNa) | 6* | 18 | Haswell, 2008; Maksaev, 2012 |
1
200 mM KCl, 90 mM MgCl2, 10 mM CaCl2, and 5 mM HEPES (pH 7.0)
2
250 mM KCl, 90 mM MgCl2, and 5 mM HEPES (pH 7.2)
3
200 mM KCl, 5 mM MgCl2, and 5 mM HEPES (pH 7.2)
4
200 mM KCl, 40 mM MgCl2, 10 mM CaCl2, 0.1 mM EDTA, and 5 mM HEPES-KOH (pH 7.2)
5
96 mM NaCl, 2 mM KCl, 2 mM CaCl2, 1 mM MgCl2, and 5 mM HEPES (pH 7.38)
6
100 mM KCl, 10 mM MgCl2, 10 mM HEPES-KOH (pH 7.4)
a
MscL is not a MscS homolog, added for reference
b
Channel activity was only shown for a V229A mutant of YbdG-encoded protein
c
number of transmembrane helices were predicted via TMHMM 2.0 server.
d
Alignments were made using Kalign algorithm in Unipro UGENE software
*
= Predicted
NR = not reported