Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Lab Invest. 2013 Apr 8;93(6):646–662. doi: 10.1038/labinvest.2013.55

Figure 4.

Figure 4

Cyp1b1−/− retinal PC display higher oxidative stress. (a) Hydrogen peroxide (H2O2) toxicity of retinal PC was measured using the MTS assay. Cyp1b1+/+ and cyp1b1−/− PC were incubated with 150 μM H2O2for 48 h and demonstrated a 1.7 -fold decrease in cell viability (***P < 0.0001). (b) Oxidative stress was measured by dihydroethidium staining in the presence of solvent control DMSO; Cyp1B1 inhibitor, TMS; antioxidant, NAC; and H2O2 for 48 h. Scale bar, 20 μm. (c) Quantitative assessment of mean fluorescent intensity is shown (*P<0.05; ***P<0.0001).