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Altered expression of ECM proteins in cyp1b1−/− retinal PC. (a) Cyp1b1+/+ and cyp1b1−/− PC were incubated for 2 days with serum -free PC medium. Cell lysates and conditioned medium were analyzed by Western blot analysis for TSP1, TSP2, fibronectin, osteopontin, and tenascin-C using specific antibodies as described in Materials and methods. (b) Quantitative assessment of band intensity was determined (N=3, *P<0.05, **P<0.001, ***P<0.0001, ****P<0.00001).
