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. 2013 Aug 15;81(3):807–818. doi: 10.3797/scipharm.1306-17

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© Salapovic et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Formula for the calculation of % of allergenic sesquiterpene lactones. A is the absorbance (A₂–A₁, see Experimental) of the test solutions at 412 nm; EW is the weight of extract (g) regarding to 1000 ml. The molar absorption coefficient of L-cysteine ethyl ester hydrochloride after reaction with Ellman’s reagent was determined to be 9900.