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The EtOH-diet (EtOH) intensifies ICAM-1 activity and gene expression at 2 h after H/R. Immunohistochemistry for ICAM-1 was performed on liver cryo-sections (panels A–D). ICAM-1 positive areas were quantified using a score, ranging from 0 (no activity) to 4 (high activity) (panel E). Hepatic mRNA levels were measured as described (F, *P < 0.05 vs. sham). n = 7–9; bar equals 50 μm.
