Figure 10.

H/R enhanced NF-κB/p65 phosphorylation and subsequent NF-κB-driven GFP expression, in mice fed the EtOH-diet. Total protein was extracted from livers of H/R mice fed the EtOH-diet, 2 h or 24 h after H/R and analysed by Western blot. In panel (A), lanes 1–2 represent mice after sham operation, or at 2 h (lanes 3–4) and at 24 h (lanes 5–6) after H/R. Densitometric measurements of GFP, (p) p65, normalized to β-actin are given (*P < 0.05 vs. 24 h H/R, sham; ^#P < 0.05 vs. Ctrl, panels B and C). n = 7–11, representative gel from 3 experiments is shown. At 2 h after H/R, MMP-9 gene expression was determined as described in Figure 5 (*P < 0.05 vs. all, panel D); n = 7–9 per group.