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Methylation-specific primed PCR. DNA methylation levels of K10 gene in differentiated HaCaT cells treated with CBD and CBG (both used at 0.5 μM), alone or in the presence of SR141716 (0.05 μM). SR141716 was ineffective when used alone. The methylation status of the K10 gene was analysed as described in the Methods section. Prol, proliferating cells; Diff, differentiated cells. ***P < 0.001 versus Prol; ###P < 0.001 versus Diff; #P < 0.05 versus Diff; &P < 0.05 versus Diff + CBD.
