Table 2.
Effects of a combination of apamin (10−8 M) and ChTx (5 × 10−8 M) on cerebrovascular responses to ACh (10−4 M) in PCA segments from WT, APP, TGF and APP/TGF mice
| Treatment | WT (n = 3) | APP (n = 3) | TGF (n = 3) | APP/TGF (n = 3) | |
|---|---|---|---|---|---|
| EAmax | Control | 45.7 ± 2.5 | 24.3 ± 2.7 | 23.4 ± 2.9 | 18.0 ± 3.1 |
| Apamin +ChTx | 15.9 ± 2.0b | 18.3 ± 3.9 | 20.4 ± 5.3 | 13.4 ± 2.0 | |
| pD2 | Control | 7.67 ± 0.14 | 8.28 ± 0.24 | 8.04 ± 0.25 | 8.02 ± 0.28 |
| Apamin +ChTx | 7.23 ± 0.28 | 7.05 ± 0.35a | 7.93 ± 0.36 | 7.98 ± 0.27 |
Data are mean ± SEM of the number (n) of mice indicated in parentheses and are expressed as the maximal agonist response (EAmax, % dilation from induced tone) or potency [pD2 value or −log (EC50)]. Apamin and ChTx significantly reduced the ACh-mediated response in WT mice, and had a small, albeit significant effect, on the potency of ACh in APP mice.
aP < 0.05 and bP < 0.001, when compared to their respective control groups before treatment, by Student t-test.