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Anti-RRV and anti-enolase antibodies cross-reactive with both RRV and enolase proteins. Western blot: Purified RRV, ovalbumin (ova), and enolase (1 μg each) were incubated with either purified anti-RRV or anti-enolase antibody, and reactivity was visualized by chemiluminescence. The ~120-kilodalton RRV protein band reactive to anti-enolase antibody was identified by mass spectrometry to contain proteins VP2, VP4, and VP6.
