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. 2013 Oct 7;8(10):e77280. doi: 10.1371/journal.pone.0077280

Figure 3. High Dietary Fat Drives a Selective Increase in the Expression of PDK4 in the Heart.

Figure 3

mRNA and protein content of PDK and PDP isoforms were measured in hearts of mice fed either the control or high fat diet for 1 d or 1 wk. A. mRNA expression determined by qRT-PCR (n = 5 and 9 for 1 d and 1 wk). B. Protein content measured by quantitative mass spectrometry (n = 5 and 6 for 1 d and 1 wk). C. Expression of PDK4 in isolated cardiac mitochondria quantified by densitometric analysis of Western blots (n = 5 for 1 wk). All data are presented as the mean ± SEM with p values: ** < 0.01; and *** < 0.001.