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. 2013 Oct 7;8(10):e77280. doi: 10.1371/journal.pone.0077280

Figure 4. High Dietary Fat Alters the Kinetics of PDH Inhibition in Wild-type but not PDK4-/- Mice.

Figure 4

Cardiac mitochondria (0.25 mg/mL) from mice fed control or high fat diets for 1 wk were incubated with 100 µM pyruvate and 1.0 mM malate. At 2.0 min, state 3 respiration was initiated by the addition of 0.25 mM ADP. PDH activity was assayed at indicated time points for A. wild-type mice (n = 5) and B. PDK4-/- mice (n = 5). C. Oxygen consumption traces for mitochondria respiring with pyruvate were recorded. The rate of state 3 respiration was calculated for wild-type mice (n = 11) and PDK4-/- mice (n = 5). D. The relative levels of PDH E1α and Hsp60 as a loading control were assessed in wild-type and PDK4-/- mice fed control or high fat diet by Western blot analysis. All data are presented as the mean ± SEM with p values: * < 0.05 and ** < 0.01.