Table 1. Effects of High Dietary Fat on Cardiac Mitochondrial Respiratory Activity.

Substrate:	Pyruvate (100 µM)		Pyruvate (1.0 mM)		Palmitoylcarnitine (25 µM)
	State 3	State 4	State 3	State 4	State 3	State 4
Low Fat:	89.0 ± 5.1***	13.7 ± 1.6	100.1 ± 7.0	18.1 ± 1.5	201.8 ± 24.2	28.4 ± 2.8
High Fat:	53.6 ± 5.9***	12.7 ± 1.7	90.2 ± 8.1	15.4 ± 0.7	217.5 ± 22.1	34.8 ± 3.3

Cardiac mitochondria were isolated from mice fed control or high fat diet for 20 wk. Mitochondria were incubated with 100 µM pyruvate, 1.0 mM pyruvate, or 25 µM palmitoylcarnitine in the presence of 1.0 mM malate. State 3 respiration was initiated by addition of 0.25 mM ADP at 2.0 min. The rates (nmol O/min/mg) of ADP-dependent (state 3) and ADP-independent (state 4) respiration are represented as the mean ± SEM with p value: *** < 0.001 (n = 9).