Table 1.

Quantitative RT-PCR Data from Normal Control Fibroblasts, AS Lymphoblasts, PWS Fibroblasts, and t(4;15) Fibroblasts^[Note]

Transcripta	NormalFibroblasts	ASLymphoblasts	PWSFibroblasts	t(4;15)Fibroblasts
SNRPN exon 2	.8 (.009)	.9 (.0004)	.0006 (.001)	2.1 (.007)
SNRPN exon 3	2.8 (.03)	2.7 (.001)	.002 (.002)	.02 (.008)
SNRPN exon 57/58	1.6 (.001)	1.9 (.001)	.001 (.001)	.01 (.001)
IPW	2.5 (.16)	2.5 (.001)	.01 (.02)	.05 (.12)
PAR-1	3.1 (.11)	2.4 (−.13)	.5 (−.3)	.3 (.005)
U21	1.5 (NC)	1.4 (NC)	.6 (NC)	1.4 (NC)
PWCR1/HBII-85	3.3 (.01)	1.7 (.04)	.01 (.02)	.01 (.02)

Note.— Data are relative number of nanograms of the transcript of interest (−RT results). C_T PCR values from amplification plots, as in figure 3, were interpreted on the basis of a standard curve and were normalized to data for β-actin for each cell type. NC indicates that all −RT reactions gave no signal and so no value was calculated (C_T >40). Primer sequences are as follows: SNRPN exon 2: 5′-ACGAACTACAGAACAGCACGTACC-3′ and 5′-CTGCGTTTGACTTGGACTTCC-3′; SNRPN exon 3, 5′-TTCTCAGCAGCAGCAAGTACCT-3′ and 5′-TGCCTCAGTTCAGCCTGGA-3′; SNRPN exon 57/58, 5′-CAGGAAAGATCAAAACGATGCA-3′ and 5′-GGCAGCTATCTGGACCAATCAC-3′; IPW, 5′-TGCATTCTTTTAGTGGATAGATGCA-3′ and 5′-TCCCCATAATGGCTTGTGTGT-3′; PAR-1, 5′-AGAGGCCAGCCATAACTAGCC-3′ and 5′-TGAAGAGCGTTCCCCTGTG-3′; U21, 5′-TGAGCAGTCAGTAGTTGGTCCTTT-3′ and 5′-GAAACAATTATCGCATCATATGCAA-3′; PWCR1/HBII-85, 5′-TCGATGATGAGTCCCCCATAA-3′ and 5′-CATTTTGTTCAGCTTTTCCAAGG-3′.

^aIPW and PAR-1 are stable transcripts from the SNRPN locus. U21 is a ubiquitously expressed nonimprinted C/D box snoRNA encoded on chromosome 1. PWCR1/HBII-85 primers recognize 4 of 27 PWCR1/HBII-85 snoRNA sequences.