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. 2013 Oct 7;8(10):e76519. doi: 10.1371/journal.pone.0076519

Table 1. Primers for RT-PCR.

Gene Sequence Ta
ANF (F) 5'-CTGCTAGACCACCTGGAGGA-3' 60°C
(R) 5'-AAGCTGTTGCAGCCTAGTCC-3'
MMP-9 (F) 5’-CACCGCTCACCTTCACCCG-3’ 66°C
(R) 5’-TGCCGAGTTGCCCCCAGTTA-3’
β-Actin (F) 5'-CATCGTACTCCTGCTTGCTG-3' 57.5°C
(R) 5'-CCTCTATGCCAACACAGTGC-3'