Table 1. Primers for RT-PCR.
Gene | Sequence | Ta |
---|---|---|
ANF | (F) 5'-CTGCTAGACCACCTGGAGGA-3' | 60°C |
(R) 5'-AAGCTGTTGCAGCCTAGTCC-3' | ||
MMP-9 | (F) 5’-CACCGCTCACCTTCACCCG-3’ | 66°C |
(R) 5’-TGCCGAGTTGCCCCCAGTTA-3’ | ||
β-Actin | (F) 5'-CATCGTACTCCTGCTTGCTG-3' | 57.5°C |
(R) 5'-CCTCTATGCCAACACAGTGC-3' |